George Combey was working as a Catholic missionary in Liberia alongside the priest who's now in isolation in Madrid.
Mr Combey died in ELWA Hospital in Monrovia, Liberia, after falling ill with the virus that has killed more than 1,000 people in West Africa.
It's thought the missionary didn't know he was infected when his friend, Fr Pajares, left, so he decided to stay in Liberia to continue his work.
Meanwhile Fr. Pajares, 75, is being treated with a new trial drug.
According to reports the priest is in a stable condition and early signs show the virus is not at an advanced stage.
Meanwhile one of the American Christian missionaries, Dr. Kent Brantly, has released a statement from his isolation room at Emory University Hospital.
Dr. Kent Brantly was ill with Ebola
He said he was growing stronger every day.
"I thank God for His mercy as I have wrestled with this terrible disease. I also want to extend my deep and sincere thanks to all of you who have been praying for my recovery as well as for Nancy (Writebol) and for the people of Liberia and West Africa.
"My wife Amber and I, along with our two children, did not move to Liberia for the specific purpose of fighting Ebola. We went to Liberia because we believe God called us to serve Him at ELWA Hospital.
"One thing I have learned is that following God often leads us to unexpected places. When Ebola spread into Liberia, my usual hospital work turned more and more toward treating the increasing number of Ebola patients. I held the hands of countless individuals as this terrible disease took their lives away from them. I witnessed the horror first-hand, and I can still remember every face and name.
"When I started feeling ill on that Wednesday morning, I immediately isolated myself until the test confirmed my diagnosis three days later. When the result was positive, I remember a deep sense of peace that was beyond all understanding. God was reminding me of what He had taught me years ago, that He will give me everything I need to be faithful to Him." He said.