The 'I will never let you down' and 'How we do' singer is taking part in a special concert before Mother Teresa is officially made a saint over the weekend.
She will sing during a concert at St Peter's Basilica.
Rita Ora recently attended the MTV Video Music Awards in Madison Square Garden, New York City.
Rita Ora, like Mother Teresa, was born to Albanian parents and moved from Kosovo to England when she was a year old.
Her mother is Catholic and her father is Muslim.
According to The Catholic Herald there will also be traditional Albanian music and sopranos such at the special event.
Rita told fans she was singing for the pope on The Tonight Show with presenter Jimmy Fallon, she said: "I'm going to do a whole 'What's up, Pope' thing."
She said she's more spiritual than religious but described the opportunity to sing for Pope Francis as "an honour".
Rita Ora will be performing the hymn What Child Is This for the pontiff.