Heroin, crack cocaine and five blades were surrendered at SPAC Nation by a "newcomer" following an altar call on Sunday, leaders said.
Senior pastor, Tobi Adegboyega said: "Our determination to clean the streets is stronger than ever before and the items handed in are a testament to that."
The knives and illicit substances are being passed on to police, according to SPAC Nation - which holds a weapon and drug amnesty every week.
Some 113 people gave their lives to Jesus when the altar call was made. SPAC Nation says 1,000 people regularly attend its weekly service.
Knives, Drugs and a phone that's used to supply drugs were all freely given up in today's service.— SPAC Nation (@SPACnation) April 14, 2019
This is what we witness in SPAC Nation when the call for change is made!#SPACNation pic.twitter.com/AMg565rMUL
Mr Adegboyega continued: "Each time we take in such items, it means one less person who will go to jail or end up dead.
"It is up to us as a community to do what we can to save these young people.
"SPAC Nation as a church and a force in the community has a dogged determination and a passion for young people to clean up the streets and give young people hope for their future.
"We will keep on doing this one knife pickup at a time."
Stay up to date with the latest news stories from a Christian perspective. Sign up to our daily newsletter and receive more stories like this straight to your inbox every morning.