In New Orleans in Louisiana, dozens gathered at a church near the French Quarter to pray for the families of the victims.
And dozens of mourners were at Metropolitan Community Church of Corpus Christi in southern Texas (above, below), where many members of the church are from the LGBT community.
Deacon Andy Wilcox, from MCC, said: "When you share a burden, it gets light."
LGBT communities across the United States and the world have been meeting after Omar Mateen, 29, opened fire at Pulse nightclub in the early hours of Sunday morning.
He declared allegiance to Islamic State in a 911 call before murdering the victims, and injuring 53 others - some seriously.
It was the worst mass-shooting in recent US history, and marked the 15th time the US President Barack Obama has had to make a public address because of a mass-shooting.
Omar Mateen's father Seddique Mateen has said the nightclub is partly to blame for his son's massacre because security wasn't good enough, and that he should've been 'neutralised' quicker.
Seddique Mateen has also said he never noticed that his son had become radicalised.