The charity has issued a stark warning that Christianity will not survive in the Middle East unless more help is sent.
Most of the money will go to refugee camps near the city of Aleppo.
Sister Annie Demerjian of the Sisters of Jesus and Mary works there and said: "If we want the Christians to remain in the Middle East, then we must help them with what they need in order to survive.
"Aleppo is facing the risk of dying a slow death."
She added that most people have "forgotten what it is like to eat meat or fresh fruit".
"Where are they going to find refuge? How are they going to repair their houses?" She said.
ACN is also supporting the Church's humanitarian work in Homs, and its surrounding towns and villages, where more than 750,000 people have been forced to abandon their homes.
Jesuit priest Fr Ziad Hilal said: "In Homs the number of people who are dependent on support for such things as foodstuffs and articles of hygiene is growing, since many people are unemployed now and have no source of income."
The extra money will mean more than 15,000 displaced persons will be provided with washing powder, soap and towels - as well as the necessities for the coming winter, such as blankets and warm clothing.