Leaders erected fencing around the statues on a lawn outside the Episcopalian Christ Church Cathedral in Indianapolis to remind people the family was "homeless" when they escaped King Herod's murderous plot by fleeing to Egypt.
Dean and rector, Rev Stephen Carlsen said: "We will not stand by while children are being taken from their parents, and families are being taken from our communities and congregations.
"People of good will and faith must not allow this to continue.
"We must not be divided by race, language or culture, but reach out to care for our neighbours-because every family is sacred."
Described as a "zero tolerance" approached, the policy was criticised for resulting in thousands of migrant children being separated from their parents at the United States' border with Mexico.
Last month, President Donald Trump issued an executive order trying to ensure incoming families were not forcibly split up.
Rev Lee Curtis, who had the idea for the demonstration, told NBC News: "Families, all families, every family, is holy, and we hope and pray that families who are seeking out a better life for their kids are afforded that opportunity."
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