More than 34,000 people around the UK signed the Christian charity's petition.
Rhian Beynon, campaign manager at World Vision UK, said in a statment: "535 million children are living in a country affected by conflict or disaster. In these crises – conflicts, natural disasters, epidemics – children and young people suffer the most while being the least responsible.
"They are particularly vulnerable to violence such as being recruited to child soldiers, sexual abuse, trafficking and hazardous labour. The UK government must step up its investment to keep them safe.
"The 34,628 supporters who have signed our petition care about protecting children around the world. They are sending an important message the Secretary of State for International Development cannot ignore."
The UK government has already pledged to end all violence against children by 2030.
However, the charity has argued that their research shows that in 2017, only 2.5 per cent of the Government's bilateral humanitarian spending went on projects safeguarding children from violence.
World Vision UK's petition, which was launched as part of the charity's It Takes A World campaign, has called on DFID to this year commit to increasing this spending to at least 10 per cent of the humanitarian budget.
"Protecting the world's most vulnerable children from violence is worth more than the current price tag the Government is putting on it," Beynon added.
"The UK Government must dramatically scale up the humanitarian aid it spends on keeping children safe. This is crucial to meet its promise to end violence against children living with conflict and disaster."
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