The Shroud is a piece of linen that is roughly three metres long and is regarded by many Christians as the ancient burial cloth Jesus was wrapped in after his crucifixion.
Experts found that the cloth contains nanoparticles which are not typical of the blood of a healthy person.
The study carried out by various institutions under Italy's National Research Council suggests the theory that Jesus' face was painted onto the cloth by forgers in medieval times is inaccurate.
Elvio Carlino, who led the research at the Institute of Crystallography in Bari, Italy, said the victim wrapped in the funeral cloth likely underwent "great suffering" before his death.
Professor Giulio Fanti from the University of Padua agreed the blood contained high levels of substances called creatinine and ferritin, usually found in people who suffer trauma or torture.
He explained: "Hence, the presence of these biological nanoparticles found during our experiments point to a violent death for the man wrapped in the Turin Shroud."
The burial cloth which bears the faint image of a crucified and beaten man, has been an enigma for centuries - being both subject to intense scientific study to ascertain its authenticity as well as an object of reverence.
The Catholic Church takes no official position on the Shroud's age but says it is a matter for scientific investigation.
Carbon tests in the 1980s dated it at about 700 years old.