The sign - given to the Pope by Italian psychologist Salvo Noe - explains "violators are subject to a syndrome of always feeling like a victim and the consequent reduction of your sense of humour and capacity to solve problems".
The notice, in large white letters on a red background, appeared in recent days on the door that leads to the Pontiff's private rooms in Casa Santa Marta, where he lives in the shadow of St Peter's Basilica.
It is not clear who the notice is aimed at but it has been suggested cardinals, priests and members of his staff could be the main target of his anti-whining sign.
For anyone caught whining in front of children, "sanctions will be doubled".
Ok, who annoyed the Pope so much he put a "No Complaining" sign on his door at the Vatican? pic.twitter.com/HGD8ku8weh
— ian bremmer (@ianbremmer) July 14, 2017
The sign continues: "To get the best out of yourself, concentrate on your potential and not on your limitations.
"So stop complaining and act to change your life for the better."
The arguably well-humoured notice corresponds with the Catholic leader's leadership style that has encouraged a more lenient approach on issues such as divorce and homosexuality.
He has also encouraged the world's billion-plus Catholics to be more joyful.
A few months after he was elected in 2013, Francis told a gathering of faithful: "Sometimes there are melancholic Christians with faces like pickled peppers, such long faces, rather than being joyful for the beautiful life they have."
