The Sistine Chapel will be closed off from the rest of the public for 90 minutes, so the group also get to visit the Vatican gardens and musuems, before having dinner afterwards.
Some 25,000 people a day, or five million people a year, visit the chapel. Entry to the Vatican Museums and Sistine Chapel costs €16 (£10), giving an annual revenue for the Vatican of around €80 million or £60 million a year.
The tour will be led tomorrow by Archbishop Konrad Krajewski, the chief alms-giver for the Vatican.
It's the latest of several gestures Pope Francis has approved towards the homeless. Willy Herteleer, a Belgian man well-known around St Peter's Square, became the first homeless person to be buried after his death earlier this year.
And recently, the homeless were given dozens of sleeping bags with the Vatican crest emblazoned on them, and hundreds of umbrellas.
Hot showers and a barber shop run by volunteer's were also set up in St Peter's Square for them.
Some shopkeepers and residents near the Vatican have complained that the number of homeless people in the area have been growing, and that they've been driving away business and leaving illegal objects and human waste there.
Some 25,000 people a day, or five million people a year, visit the chapel. Entry to the Vatican Museums and Sistine Chapel costs €16 (£10), giving an annual revenue for the Vatican of around €80 million or £60 million a year.