The Christian charity is donating money and supplied to help the town of Slovyansk which has been heavily damaged by fighting between pro-Russian rebels and Ukrainian forces.
The organisation has delivered a 15-ton truck with emergency essentials to distribute to local residents including clothing, shoes, nappies, bedding and bottled water.
It also brought roofing materials and supported others with the delivery of more than 65 tonnes of food to people who had been unable to leave the town.
Local community groups are being also offered help including Christian builders and money is being given to local churches to repair buildings.
Mission Without Borders' manager in Ukraine Mykola Bohdanets said: "The situation is slowly changing for the better. The people of this town have been hit by the horror of war and we were honoured to have the opportunity to support them in starting to put their lives back together.
"We've worked closely with local churches to serve people's practical needs, thus both opening hearts to hear God's message of love and bringing hope back to the fearful people of Slovyansk".