Christian Aid has allocated more than £75,000 to provide emergency assistance following the heavy rains which started in June and have worsened over the last few days.
Shivani Rana, Emergency Programme Officer at Christian Aid said: "The numbers of people who need help are enormous and now persistent and very heavy rains are forecast for the coming days so numbers will grow.
"Villages have been wiped out by the flood water. People need shelter, clean drinking water and food."
Through the charity's local partner organisation - Indo Global Social Service Society - shelter items such as rope, water filters, soap and mosquito nets have been distributed.
The rains have burst the banks of rivers, flooding villages and agricultural land across 15,000 hectares throughout Assam State.
Toilet facilities are also underwater and the risk of disease spreading throughout the area is extremely high.
Rana added: "Getting access to those who need help is almost impossible because the roads are submerged so we have to rely on our partners' local knowledge and use alternative routes".
Relief camps have been set up by government authorities and around 22,000 people are taking shelter in them.