Priests from the Diocese of Rochester, New York State, have been told they may not travel to West Africa.
Bishop Salvatore Matano said it was to stop the spread of the deadly Ebola virus.
Five thousand people have died from the illness which is rapidly spreading across Liberia, Guinea and Sierra Leone.
Any priest who ignores the ban may be stopped from working in the diocese on their return.
"I hope this decision does not cause any grave inconvenience but the seriousness of the Ebola epidemic demands a strong and reasonable response," said Revd Edward Palumbos, the diocese director of priest personnel.
He added: "The bishop deeply appreciates your many contributions to the life of our diocese and is extremely concerned that neither you nor the people with whom or to whom you minister fall victim to this disease.
"This decision is being made purely from a pastoral concern for the people of God here in Rochester and to insure that they maintain the highest confidence in us who minister to them in Christ's name."
It's thought other Bishops in New York State could follow this rule.
A Monthly Gift Of $11 Makes A World Of Difference
In a world of fake news there’s never been a greater need for quality Christian journalism. Premier’s mission is to provide the Church with the most up to date and relevant news, told from a Christian perspective. But we can’t do it without you.
Unlike many websites we haven't put up a paywall — we want to keep our journalism free at the point of need and as open as we can. Premier’s news output takes a lot of time, money and hard work to produce. No one in the USA is sharing news like we are across radio, magazines and online so please help us to continue that today.
For a monthly gift of $11 or more we’d also be able to send you a free copy of the brand new Premier Bible, a wonderful Anglicised version of the NLT packed with exclusive bonus content, reading plan and resources to help you get the most out of scripture.
Your monthly support will make a world of difference. Thank you.