Dennis Sheehan, who's been described as a 'calm and kind Christian man' was in his late sixties passed away at a hotel in Los Angeles while on tour with the band.
Speaking of his death, lead singer Bono said: "We've lost a family member, we're still taking it in.
"He wasn't just a legend in the music business, he was a legend in our band. He is irreplaceable"
Rick Warren took time to pray with the band and read the Bible before their concert last night.
Writing on his Facebook page, he asked believers to remember the band as they mourn. He said: "U2's road manager for 33 years, the amazing Dennis Sheehan died suddenly last night here in LA. Dennis was a calm and kind Christian man.
"The team is heartbroken so Adam, Larry, Edge, and Bono asked me to share some scripture, words of comfort, and pray with them right before they walked on stage tonight.
"After sharing the '#ChooseJoy' story from my son's death, I asked "So how do you go out and do a concert when your heart is hurting?" Bono said "We choose joy!" Right. Pray for Pam who lost the love of her life.
Rick Warren's wife Kay wrote the book 'Choose Joy' and used the slogan when dealing with her son's death in 2013.