It comes after an increase in attacks against Christians in the south Indian state of Telangala.
International Christian Concern (ICC) says the protest started after a group of more than 100 Hindu nationalists allegedly attacked a Christian gathering, beating Christians with iron rods and knives.
Three pastors required critical medical attention, according to ICC.
Days before the protest, the United States Commission on Religious Freedom ranked India in the second worst tier for Christian persecution in the world.
But the Indian government has rejected the Commission's report.
Solomon Raj, from the Twin Cities Christian Youth Fellowship in Telangala, was part of the protest.
He claims extremists affiliated with Narendra Modi's ruling Hindu nationalist BJP Party attacked a carol service last Christmas, kicking a pastor in the middle of a road and destroying his bible.
Speaking of the protest, he told Premier's News Hour: "Besides the Christians... the Muslim prominent leaders - even the Hindu peoples also attended, to show the solidarity to the Christians.
"[The] government is saying that India is a Hindu country, whereas Article 25 in the Indian Constitution says that... everybody has the right... [to] perform their religion. It is in the constitution, but the [Prime Minister Narendra] Modi government is not following this.
"It was a very big event on Saturday because most of the people, all the Christian churches and organisations, they came forward in one... under the one roof, and they have given a very big protest, and I think the government should react on this and they have to protect the Christian people, they have to give security to the Christian people.
"Many pastors and believers are attacked continuously around the nation."
Listen to Solomon Raj speaking to Premier's Des Busteed on the News Hour: