It is believed that the King James is the most read English book.
American scholar Jeffrey Miller found the book, dating from 1604 to 1608, in an archive at Sidney Sussex College.
Some pages had illegible handwriting whilst others included notes on Biblical stories in Greek and Hebrew.
Nicholas Rogers is an Archivist at Sidney Sussex College, Cambridge and told Premier's News Hour it was a significant discovery.
"It certainly is the most important thing we've discovered in our archive since I came in here in 1989," he said.
He added: "This notebook indicates that each member of the company was assigned a particular book and they all translated them on their own."
Premier's Hannah Tooley speaking to Nicholas Rogers:
