Complaints regarding the LGBT rules at Citipointe Christian College have been relayed to the Queensland Human Rights commission.
The college found themselves embroiled in controversy in early February when they sent contracts to parents comparing homosexuality to bestiality and paedophilia.
The contracts - sent ahead of the start of the Australian school year - asked parents to denounce homosexuality and would only allow young people to be enrolled under their biological sex.
Labelled "a statement of faith", parents were expected to sign the contracts to ensure their ethics aligned with the Christian institution.
After causing global outcry, the contract was revoked. A short time later, the school's principal, Brian Mulheren, resigned from his position.
However, before the statement was removed, parents referred the matter to the Non-State School Accreditation Board.
Parents are now taking legal action against the institution.
Elise Myers is one of the people pushing the discrimination complaint. She told Australia's ABC News:
"The deeper issue is putting policies and procedures in place so that this doesn't happen again, and so that we create an environment that's safe and inclusive for all students."
Ms Myers' children still continue to study at the school.
She continued: "If you think about what our core values are in sending our children to a Christian school, we think of those big values of love and respect, equality, justice, and this kind of discrimination obviously does not fit anywhere in those."