The register lists more than five thousand places of historic significance that are at risk because of neglect or overdevelopment.
Churches now on the list include the church where William Wilberforce worshipped and met with others to plan the anti-slavery campaign.
The rector of St Mary Woolnoth Church in London at the time was John Newton, the author of Amazing Grace.
The church has been flagged by Historic England due to damage to it's roof and cracks in it's tower.
It was built at the beginning of the 18th century and designed by Nicholas Hawksmoor after the Great Fire of London.
Jeremy Crossley is the vicar at the church and told Premier about his mixed feelings at appearing on the list: "I'm relieved that it's been recognised as an important building but in need of help.
"But also anxious because, ever since I became the vicar three or four years ago, I've been aware of the problems and we've been raising money and trying to do something each year to deal with all that's needed to be done."
The church needs just over £2million worth of restoration to be completed on the building.
Reverend Crossley added: "There's some quite fundamental work that needs to be done, as well as some restoration of the interior to the original Hawksmoor design so it will be not only safe and secure for future generations but will also reflect the original vision of the building which is particularly relevant to contemporary worship here in the city of London".
Buildings included on the Heritage at Risk Register may be seeking grants for essential repairs.
Some of these will be available from Historic England, the Heritage Lottery Fund and Natural England, but Duncan Wilson, the chief executive of Historic England, said there is "a significant challenge in saving these sites for future generations".
One the biggest medieval parish churches in England, the Grade I listed Holy Trinity Church in Hull, is among other sites included on the list.
Dan Golding