Christine Weick took offence at an individual dressed as a giant rabbit and taking part in a fun day at a church in Tennessee.
"This is a church of the holy God. You are nothing more than Santa Claus coming to a church. Shame on you," she screamed.
The woman is well known for confrontation. She had once challenged the father of pop star Katy Perry about his daughter's music videos.
In her latest recording she is seen driving to the church before confronting the bunny actor, who continues to wave at her whilst she shouts.
"Call yourself a Christian in a pagan costume?" She asked.
"When you stand before God, I wonder if you'll be wearing that," she said.
At one point, a friend of the woman can be heard screaming "Hugh Heffner, playboy bunny!"
The pastor of the church said to the woman "you all have a good day" as he asked her to leave.
"Get a life," he said, to which she replied: "My life is with the Lord Jesus Christ who died on the cross, not some stinking bunny".