He and his sister Ann Gloag generated or donated more than £20 million for mainly social, medical and Christian causes.
The Sunday Times Giving List ranks celebrities on the proportion of their income they give to charity.
Sir Elton John and JK Rowling topped the list giving away more than £37 million between them in the past year.
The Sainsbury family topped the giving list for the third consecutive year. They gave away £220 million through a network of 18 trusts, equivalent to 40% of their residual wealth.
Sir Brian Souter runs his own charitable trust and is open about how important his Christian faith is to him.
His website says he is often asked what makes him tick and replies that it's not wealth or riches and instead his "deep-seated Christian faith".
He's a member of the evangelical Church of the Nazarene.
The Scotsman is also a prominent supporter of the Scottish National Party and has made several donations to the organisation.