Fr Rubén Alcántara DÃaz, 50, judicial vicar at the church of Nuestra Señora del Carmen, was stabbed to death last Wednesday evening, just minutes before the 7pm Mass he was due to celebrate.
On Friday, Fr Juan Miguel Contrera Garcia, 33, was shot dead by a group of armed assailants in his parish in the State of Jalisco.
The attack reportedly took place immediately after he had celebrated Mass.
According to the Catholic Multimedia Center, at least four priests have been slain in Mexico this year, and 23 have been murdered since 2012.
Responding to the latest deaths, Cardinal José Francisco Robles Ortega, Archbishop of Guadalajara said in a statement: "We make an urgent call to build a culture of peace and reconciliation. These lamentable events call all of us to a much deeper and sincere conversion.
"It is time to look honestly at our culture and society, and to ask ourselves why we have lost respect for life and for the sacred."
The cardinal also called on the authorities to investigate the crimes and not allow it to go unpunished.
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