The Baptist Union of Great Britain, the Church of Scotland, the Methodist Church, the United Reformed Church and Quakers in Britain all say the proposals to renew the weapons system are unnecessary.
The call to stop investing in the technology comes ahead of a scheduled debate in parliament.
The UK government wants approval to spend £41 billion on building special new submarines to carry the weapons.
Previously the faith groups have spoken out against the renewal suggestions and have criticised the government for pushing back against United Nations discussions on multilateral disarmament.
Churches have been represented at Non-Proliferation Treaty conferences in the USA and have been trying to influence government policy on nuclear weapons for years.
Last year 26 faith leaders publically asked the UK government to join other nations to help create a global plan that would free the world of all nuclear weapons capabilities.
The leaders of the five Churches, that have more than a million members in the UK, are also asking Christians to pray and to write to their MPs asking them to push for change.
Rachel Lampard, Vice-President of the Methodist Conference said: "It is scandalous that the UK Government has consistently opposed opportunities for discussion on multilateral disarmament.
"A decision to build Trident submarines now, just as talks on disarmament are due to get underway in the United Nations General Assembly, seems ill-timed and unwarranted."
Revd Stephen Keyworth, Faith and Society Team Leader, the Baptist Union of Great Britain commented: "We have an opportunity to give strong moral leadership and to work creatively as peacemakers.
"We will never achieve the peace which Scripture encourages us towards with a defence policy built on fear -peace is achieved through justice and relationship, not fear."
