After a two-and-a-half-year build-up, Scots will go to the polls on Thursday to decide if Scotland should leave the United Kingdom.
More than four million people will be asked 'should Scotland be an independent country?' at the ballot box.
At Sunday services prayers were said for God's guidance ahead of the vote and churches across Scotland have been invited to stay open for the duration of polling day.
It is hoped that practising and non-practising Christians will take the opportunity to pray ahead of making the decision.
Free Church of Scotland Rev Dr Colin Dow's church on St Vincent Street in Glasgow is one of the churches that will remain open.
He told Premier it was important that people properly thought through the issues before casting their ballot.
He said: "It's to make sure we're discerning the will of God in this situation but also understanding more about the issues that are at stake and bringing them before God, and that can't be a bad thing for any of us.
"And also for the result when it comes through on Friday, that there will be reconciliation."
Each hour between 7am and 10pm, churches that are open will say the Lord's Prayer for an "outcome that benefits Christ's kingdom in Scotland".
First minister Alex Salmond, from the Yes campaign, and leader of the Better Together side, Alistair Darling, have been invited to attend any church to pray during the day.
It is not yet known if they will take up the offer.
Also taking part in the day of prayer are: the Church of Scotland, Catholic Bishops' Conference of Scotland, Scottish Episcopal Church, Baptist Union of Scotland, United Free Church of Scotland, Elim Pentecostal Church, Salvation Army, Destiny Church Network, Church of the Nazarene, Assemblies of God, Methodist Church in Scotland, the Apostolic Church, Pray for Scotland, Parliamentary Prayer for Scotland, King's Church Edinburgh (New Frontiers) and many other individual churches.