Churches, mosques and temples have until 29 May to apply for the Places of Worship Security Funding Scheme.
The funding can be used to pay for security measures including CCTV, alarms, external lighting and perimeter fencing.
To qualify, applicants must prove that their place of worship is vulnerable to hate crime.
Last November 45 churches, 12 mosques, one Hindu temple and one gurdwara were granted funding in the first round of the scheme.
One church in the West Midlands was awarded just over £10,000 for CCTV, intruder alarms and perimeter fencing.
Sarah Newton, Minister for Vulnerability, Safeguarding and Countering Extremism, said:
"Hate crime has a devastating impact on individuals and communities. It has absolutely no place in a Britain that works for everyone, and this Government is determined to stamp it out forever.
"For people of all faiths, right across the UK, their place of worship is a refuge - and an attack on that place of safety can be deeply upsetting.
"This money will help prevent hateful attacks on our places of worship, and will make it easier to prosecute these despicable crimes when they do happen."