Last year, MPs accused Sports Direct of running its warehouses like a "Victorian workhouse" after workers complained of poor working conditions which saw some members of staff being paid below minimum wage.
Sports Direct says it has since made steps to improve health and safety.
Church of England, Methodist and Catholic clergy from Shirebrook have come together to offer the chaplaincy service every Monday.
The three churches are part of an ecumenical group that approached company bosses with the idea.
Sports Direct have set aside an area of the staff canteen for clergy to meet with staff to discuss personal and work issues.
The company is the biggest employer in the area with more than 4,000 staff employed at Shirebrook.
Rev Karen Bradley of Holy Trinity church in Shirebrook said: "We're offering a friendly, confidential support service independent of Sports Direct, and we don't share the content of our conversations.
"We're here to provide a listening ear and give help and direction to those who need it. It doesn't need to be a work related issue, or a faith related issue, we can help with family, financial or relationship concerns."