White paint was thrown in the grounds, causing damage to the car park of Crumlin Presbyterian on Main Street.
Police confirmed they were treating it as a sectarian hate crime.
PSNI Sergeant Chris Tate said: "Some time between Saturday lunchtime and Sunday morning, white paint was thrown in the grounds of the church, causing damage to the car park area.
"The incident is being treated as a sectarian hate crime and I would appeal to anyone who has any information about the incident or who saw any suspicious activity in the area to contact officers in Antrim."
People with information relating to the incident should call 101 quoting reference number 567.
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