The 155m long boat has been built by Australian creationist Ken Ham.
Mr Ham is the head of the group Answers in Genesis and says that the ark is built based on dimensions given in the Bible.
Inside the ark are images of Noah's family and cages containing toy animals and dinosaurs.
The project has been in the planning in 2010 and has fallen under heavy criticism by some who argue that it undermines science education.
One group against the construction, the Tri-State Freethinkers, said: "Basically, this boat is a church raising scientifically illiterate children and lying to them about science."
But Ken Ham told the Daily Mail it will be one of the "greatest Christian outreaches of this era in history."
It has been constructed mostly from wood and Mr Ham estimates around two million visitors will attend in the first year.
