Mary Jones World stands on the edge of Bala Lake in North Wales.
The centre will give people the opportunity to discover the story of Mary Jones and Thomas Charles, and to learn about the impact of the world's bestselling book, not only in Wales, but across the rest of the world through engaging and interactive materials.
In 1800, a 15-year-old Mary walked 26 miles from Llanfihangel-y-Pennant to Bala to purchase a Bible from Rev Thomas Charles.
She had saved for six years and Rev Charles was so moved by her determination that he helped establish Bible Society four years later.
Speaking ahead of Sunday's opening, Bible Society chief executive, Paul Woolley, told Premier's News Hour: "We don't want this to be a museum for the past.
"It's about the significance of the Bible in the world today.
"We want people, through the story, to discover something of the significance and relevance of the Bible for our own society, the wider world and for ourselves as individuals."
The centre can be visited by anyone during October with a special reduced rate of £3 for adults and £1 for children.
From November to March, the centre will be open to school visits and group bookings only.
The centre will reopen to the public in April 2015.